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. Author manuscript; available in PMC: 2015 Dec 1.
Published in final edited form as: Mol Cancer Ther. 2014 Sep 25;13(12):3024–3036. doi: 10.1158/1535-7163.MCT-14-0400

Figure 2. Rapamycin modulates the expression of costimulatory molecules and cytokine secretion from Flt3L-induced DCs.

Figure 2

(a) Maturation markers on Flt3L-induced rat bone marrow DCs (gated using forward and side scatter profiles) were analyzed by flow cytometry after 7 days culture (iDCs) or after stimulation with media (mock) or 50 ng/ml LPS or 50 ng/ml LPS + 10 ng/ml Rapamycin (LPS + Rapa) for 48 hrs. Overlays from one representative experiment are depicted. Results in (A) are represented as fold change in mean fluorescence intensity (MFI). (b) DCs harvested after 7 days in culture were treated with either 100 ng/ml Rapamycin (Rapa) or saline (mock) in the presence or absence of LPS for 48 hrs. Cytokine secretion was measured by ELISA. TH1/TH2 cytokine balance was estimated by IL-12 vs. IL-10 cytokine ratio. Data in (a) and (b) was analyzed using One-way ANOVA followed by Bonferroni’s post-test and are expressed as mean ± SEM, * p < 0.05, ** p < 0.01, *** p < 0.005, ns not significant.