Fig. 2.
Recovery of hPreP activity by reduction. Four h oxidized hPreP, 2.4 μM, was incubated for 3 h at 37 °C with the indicated additions and then assayed for activity. The activity of unoxidized hPreP was set to 100%. When added, DTT was 10 mM and methionine sulfoxide reductase A was 1 μM. Panels A and B are results from two separate preparations of hPreP. The results are plotted as the mean and SEM with n=4 in panel A and n=3 in panel B. Comparisons were assessed by Student’s t test with the Bonferroni correction for multiple comparisons. Pairs whose p values are less than 0.05 are marked with an asterisk, and those whose p values were ≥ 0.05 are marked n.s. (not significant).