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. Author manuscript; available in PMC: 2015 Dec 1.
Published in final edited form as: Free Radic Biol Med. 2014 Sep 16;0:57–63. doi: 10.1016/j.freeradbiomed.2014.08.016

Fig. 3.

Fig. 3

Oxidation of hPreP induces oligomerization whose extent matches the loss of activity. (A) Non-reducing SDS-PAGE gel of hPreP subjected to varying treatments. 1 μg hPreP was loaded in each lane. Oligomerization was induced by hydrogen peroxide and reversed by subsequent incubation with DTT. (B) Oligomerization and inactivation are quantitatively linked. hPreP was oxidized in 50 mM HEPES at varying pH. Regression lines fit separately to oligomerization and inactivation did not differ from each other (p=0.62) and thus a single regression line is shown.