Fig E1.

Representative standard concentration curve for the IgG anti-IgE ELISA using omalizumab (A). Comparison of the capacity of IgE-specific IgG autoantibodies to bind to “free” IgE (dark bars) and FcεRI-bound IgE (white bars) # analyte below detection limit of assay. Bars show mean/SD of 3 experiments using duplicate samples (B). SDS-PAGE showing the purified IgG anti-IgE from serum compared with recombinant IgE and IgG (C). Representative standard concentration curve for the IgG anti-FcεRI-bound ELISA using anti-IgE purified from human sera (D).