Fig E4.

Response of blood basophils stimulated with Der p 2 (3 to 300 ng/mL), pre-incubated with 4 sera containing IgG anti-IgE autoantibodies and basophil-activating activity (A) and 3 sera containing IgG anti-IgE autoantibodies without basophil-activating activity normalized to baseline (pre-incubation of the cells with the donor's own serum) (B). Effects of the non-inhibitory serum NAA7 following the removal and purification of IgE binding proteins on Der p 2 (30 ng/mL)–induced basophil activation (C). Bars represent the mean/SD of 3 independent experiments. ∗P < .05, ∗∗P < .01, and ∗∗∗P < .001.