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Binding of recombinant Phl p 7-specific IgE to FcεRI on RBL-SX38 cells (A). Phl p 7 binding following further incubation with specific allergen (representative of 3 independent experiments) (B). Changes in surface-bound IgE (C) and surface-bound Phl p 7(D) on RBL-SX38 cells pre-incubated with recombinant, anti-Phl p 7 IgE test sera then Phl p 7 compared with no serum control. Bars represent the mean/SD of 3 independent experiments. ∗∗P < .01 and ∗∗∗P < .001.
