Fig E9.

Detection of ST2⁺ cell populations in bleomycin (BLM)–induced fibrotic lung tissue. Mice were given intranasal PBS or bleomycin on day 0, and lung tissues were harvested on day 7. Tissues were dispersed, and live single-cell preparations stained with fluorescently labeled antibodies to ST2 and different cell linage markers, as described in the Methods section. Fluorescence-activated cell sorting dot plots and histograms are presented. ST2 expression was detected on macrophages (CD11b⁺F4/80⁺; Fig E9, A), dendritic cells (CD11c⁺, conventional DCs [cDCs], CD11b^dim and resident/plasmacytoid dendritic cells [pDCs]; Fig E9, B), granulocytes (neutrophils [NOS] and eosinophils [EOS]), and lymphocytes (Lymphs), natural killer (NK) cells, and natural killer T (NKT) cells (Fig E9, D). Data are representative of 2 experiments (n = 5-6 mice per group per experiment).