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. 2014 Dec 8;4:7306. doi: 10.1038/srep07306

Figure 1. Allosteric thermodynamic cycle and the CHESCA experimental design.

Figure 1

(A) Allosteric thermodynamic cycle of PKA RIα based on the coupling of auto-inhibitory and cAMP-binding equilibria. (B) Chemical library selection for the CHEmical Shift Covariance Analysis (CHESCA). The blue circles mark cAMP sites that interact directly with the CBD and are modified in the CHESCA library (e.g. Sp-cAMPS, Rp-cAMPS and 2′-OMe-cAMP). (C) Representative HSQC peaks of two residues, i and j, subject to the CHESCA perturbations outlined in (B). (D) The corresponding pairwise inter-residue correlation plot for the compounded chemical shifts (CCS) of residues i and j.