Figure 1.

The Rps19 knockdown mouse model and the design of lentiviral vectors. (A) Overview of the modified loci. (B) Breeding strategy to adjust the level of Rps19 downregulation. (C) Overview of the generated vectors. (D–E) c-Kit–enriched hematopoietic progenitors (0.25×10⁶) from the BM of uninduced mice were transduced and seeded in liquid cultures in the presence of doxycycline. (D) Cell counts on day 4. (E) Expression of endogenous Rps19 and vector-derived RPS19 on day 4. Data shown in (D) and (E) represent the average of two independent experiments with two technical replicates each. SA: splice acceptor; pA: polyadenylation signal; RSV: Rous sarcoma virus; ψ:packaging signal; SD: splice donor; RRE: rev-response element; cPPt: polypurine tract. Black arrowheads in panel (A) indicate transcriptional start sites.