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. Author manuscript; available in PMC: 2014 Dec 8.
Published in final edited form as: AIChE J. 2014 Apr;60(4):1225–1235. doi: 10.1002/aic.14351

Figure 2.

Figure 2

Schematic representation of the experimental plan. Undifferentiated hESCs were cultured either on 2D TCPS or in 3D microwells. After 6 days in culture as undifferentiated cells, colonies were enzymatically detached and placed in suspension to initiate EB formation and differentiation. EBs were collected immediately after removal from the substrate and every 24 hours following for data analysis to compare differences between EBs generated from 2D and 3D microwell culture of undifferentiated hESCs.