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. Author manuscript; available in PMC: 2014 Dec 8.
Published in final edited form as: AIChE J. 2014 Apr;60(4):1225–1235. doi: 10.1002/aic.14351

Figure 5.

Figure 5

(A) Quantification of representative EMT-related gene expression agarose gel bands, relative to GAPDH and ACTB, in undifferentiated hESCs cultured for 6 days on 2D TCPS (light) and in 3D microwells (dark) (B) Average intensity of E-cadherin fluorescence per cell, determined by flow cytometry, in undifferentiated hESCs cultured on 2D TCPS and in 3D microwells. (C) Quantification of western blot intensities of E-cadherin relative to β-actin in biological replicates of hESCs cultured for 6 days on 2D TCPS or in 3D microwells. Sample western blots representative of data are shown (• indicates p<0.05 compared to same-day 2D control)