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. Author manuscript; available in PMC: 2014 Dec 8.
Published in final edited form as: Environ Microbiol Rep. 2012 Mar 12;4(3):307–315. doi: 10.1111/j.1758-2229.2012.00334.x

Figure 1. Intergroup interindividual variation in the diversity and relative abundance of Archaea 16S rRNA gene and mcrA TRF.

Figure 1

A nested PCR-TRFLP approach was used for the amplification of Archaea 16S rRNA gene and mcrA from stool samples from healthy native Africans (NA; n = 19), African Americans (AA; n = 18) and European Americans (EA; n = 17) taken from fresh feces voided during the first defecation of the morning and immediately stored under anaerobic conditions at −80°C until DNA extraction was performed. Genomic DNA was extracted from 200 mg of stool sample using a commercial kit (QIAamp DNA Stool Mini Kit, Qiagen, Valencia, CA). Methodological details for the nested PCR-TRFLP analyses are described in Nava et al. (2011). All primer pairs used for nested PCR are listed in Table S1.

Relative abundance of (A) Archaea 16S rRNA gene and (B) mcrA TRF in stool samples from NA, AA and EA using BfaI endonuclease for Archaea 16S rRNA gene and Hpy188III endonuclease for mcrA. Each color represents a TRF and its relative size within each chart represents relative abundance; the black bars represent the TRF that were represented more often in NA.