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. 2014 Nov 24;14:454. doi: 10.1186/1472-6882-14-454

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© Yamanaka et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Effect of heat treatment of KAOD on hepatoprotection. Serum and livers were isolated from ICR mice fed heat-treated AIN-93G or heat-treated KAOD ad libitum from day 7. (A) The activities of ALT, AST, and LDH in serum were assessed using commercially available kits. Data are presented as mean ± standard deviation, n =6. Significant difference between heat-treated AIN-93G and heat-treated KAOD: *p <0.05; **p <0.01. (B) HE stained liver sections from mice administered (a) heat-treated AIN-93G with CCl₄, and (b) heat-treated KAOD with CCl₄ were observed under a normal light microscope. Scale bar =100 μm.