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. 2014 Dec 3;14:904. doi: 10.1186/1471-2407-14-904

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© Yan et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Overexpression of E2F1 decreased GAX and TAp73, and increased MDR1, MRP, ZEB1, and ZEB2 protein expression. A, B: Protein expression levels of MDR1, MRP, TAp73, GAX, ZEB1, and ZEB2 were determined by western blotting. C, D: Reciprocal immunoprecipitation assays and western blot analysis of endogenous E2F1 and MRP interaction in SGC7901/DDP cells. E: In vitro pull-down assays using 6xHis-tagged MRP (His-MRP), GST-E2F1 and GST.