Figure 5.

Time-lapse of type VI (non-embryogenic pollen). Development shown by synchronously acquired DIC and fluorescence images of GFP. Green arrows indicate vegetative-like nucleus and its daughter nuclei. White arrows indicate generative-like nucleus and its daughter nuclei (A,B). Asymmetric division resulted in a large vegetative-like and a small generative-like cell. (C,D) First mitosis of the vegetative nucleus; the spherical generative-like nucleus remains fixed opposite to the pollen aperture. (E,F) First mitosis of the generative-like cell. (G,H) Second symmetric and synchronized mitosis of the nuclei originated from the generative-like cell. Note that the nuclei derived from the generative-like nucleus are much smaller than those derived from the vegetative-like nucleus. Gn, generative-like nucleus; PA, pollen aperture; V, vacuole; Vn, vegetative-like nucleus. Bar = 20 μm.