Figure 2. SMase-induced increase in diaphragm oxidants is prevented in p47^phox deficient mice.

Image illustrates expression of p47^phox (~44 kDa; see Fig. 1) in whole-diaphragm lysates of wild type (WT) mice and p47^phox deficient mice. Data are mean ± SE of arbitrary units of dichlorofluorescein (DCF) fluorescence in diaphragms bundles (n = 5–7 mice/group). B) Image illustrates expression of superoxide dismutase isoform 1 (SOD1; cytosolic, Cu/Zn-dependent) and isoform 2 (SOD2; mitochondrial, Mn-dependent), and catalase in whole-diaphragm lysates. We used total protein (whole lane) for normalization of western blot data. The protein gel band corresponding to ~40kDa is shown as representative of relative protein content for each lane. Catalase and SOD1 blots are normalized to same protein gel. SOD2 is normalized to a different protein gel with similar loading. Data are mean ± SE of western blot signal normalized to total protein and expressed relative to mean of WT group (fold change). * P < 0.05