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Effect of constrained indazole-based 4EGI-1 mimetic on the expression of mTOR. CRL-2813 were treated with DMSO, (E)-4EGI-1 or 1d at final concentration of 50 and 25 μm for a time period of 8 hrs and lysates were immunoblot with antibodies specific to mTOR and β-actin.
