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. Author manuscript; available in PMC: 2015 Mar 3.
Published in final edited form as: Chembiochem. 2014 Jan 23;15(4):595–611. doi: 10.1002/cbic.201300723

Figure 4.

Figure 4

Effect of constrained indazole-based 4EGI-1 mimetic on the expression downstream effectors of mTOR. CRL-2813 were treated with DMSO, (E)-4EGI-1, 1a, 1l or 1d at final concentration of 50 and 25 μm for a time period of 8 hrs. Changes in the expression of p70S6K, phosphorylated- and total-4E-BP1 (P- and T-4E-BP1, respectively) are shown. Immunoblot for β-actin is shown as a control for total protein in the extract, as well as evidence for lack of effect of these compounds on housekeeping proteins.