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Effect of (E)-4EGI-1 and compounds 1a, 1l, and 1d on the ratio of levels of phosphorylated 4E-BP1 (P-4E-BP1) to levels of total 4E-BP1 (T-4E-BP1) in CRL-2813, at final concentrations of 25 and 50 μm. Blots from two independent experiments were quantified using Odyssey software. The compounds reduced the levels of phosphorylated 4E-BP1 relative to the total amount.
