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. Author manuscript; available in PMC: 2014 Dec 8.
Published in final edited form as: Science. 2010 Jan 28;327(5968):973–977. doi: 10.1126/science.1183147

Fig. 3.

Fig. 3

AcN-degrons in yeast proteins. (A) Lanes 1 to 3, CHX chase for 0, 1, and 2 hours in wild-type S. cerevisiaeexpressing Tbf1ha. Lanes 4 to 6, 7 to 9, and 10 to 12, analogous patterns but in doa10Δ cells withMKTbf1ha (Lys at position 2), GTbf1ha(initially MGTbf1ha), and wild-type Tbf1ha, respectively. (B) As in (A) but with wild-type Slk19ha and its mutant derivatives in wild-type versus doa10Δ cells. (C) As in (A) but with wild-type Ymr090wha and its mutant derivatives in wild-type versus doa10Δ cells. (D) As in (C) but with wild-type Ymr090wha in wild-type versus doa10Δ and ard1Δ cells. (E) As in (A) but with wild-type His3ha in wild-type versus doa10Δ cells. (F) As in (E), with wild-type His3ha in wild-type versus doa10Δ and ard1Δ cells. (G) As in (A) but CHX chases for 0, 0.5, 1, and 2 hours with wild-type Aro8hain wild-type versus doa10Δ and ard1Δ cells. (H) As in (A) but with wild-type Hsp104ha and its mutant derivatives in wild-type versus doa10Δ cells.