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Rigidly linked smart tetrazine probes: tetrazine-functionalized (A) BODIPYs (36–38), (B) coumarins (39–42), and (C) xanthenes (43–44). Spectroscopic properties of compounds 36 to 42 correspond to the products derived from cycloaddition with trans-cyclooctene. For compounds 43 and 44, spectroscopic properties correspond to products derived from cycloaddition with cyclopropene or with trans-cyclooctene (and subsequent oxidation), respectively.
