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Phosphine probes activated by ester cleavage in the Staudinger ligation. (A) A FRET-based smart phosphine probe (9) where a quencher is released upon Staudinger ligation. Spectroscopic properties correspond to the product derived from reaction of 9 with benzyl azide. (B) A caged luciferin-phosphine conjugate (11) that releases free luciferin upon Staudinger ligation.
