Table 2. Biochemical analysis of MEC-17 Mutants.

The Michaelis-Menten K_m and V_max values were determined by direct fitting of linear initial velocities determined at substrate concentrations of 1-20 μM. Error values represents standard error.

Biochemical Analysis of MEC-17 Mutants
Mutation	Km (μM)	kcat ×10-2 (sec-1)	kcat / Km ×103 (sec-1M-1)
WT (1-236)	13.64±2.70	3.79±.39	2.78±.84
WT (1-193)	45.37±55.76	3.24±3.03	0.71±1.53
Q58A	N/A	N/A	N/A
L60A	N/A	N/A	N/A
I64A	N/A	N/A	N/A
S66A	84.26±66.24	9.23±6.16	1.10±1.59
R69A	98.42±48.78	2.76±1.18	0.28±0.26
N73A	23.39±3.24	4.91±.43	2.10±0.47
K98A	N/A	N/A	N/A
K102A	N/A	N/A	N/A
K103A	31.24±18.55	2.79±1.15	0.89±0.61
L104A	N/A	N/A	N/A
F105A	N/A	N/A	N/A
C120A	N/A	N/A	N/A
L122A	N/A	N/A	N/A
Q131A	14.64±4.06	2.75±1.2	1.88±1.34
R132A	52.03±29.31	3.32±1.46	0.63±0.92
D157A	N/A	N/A	N/A
D157N	N/A	N/A	N/A
R158A	79.48±67.3	4.81±3.42	0.61±0.94
P159A	36.88±18.73	.97±.35	0.26±0.23
S160A	50.94±22.51	3.67±1.26	0.72±0.56
K162A	48.4±13.66	6.49±1.46	1.34±0.68
L164A	3.45±5.25	.56±.12	1.62±2.81
K169A	43.28±20.78	3.45±1.25	0.80±0.67
L173A	36.75±54.19	1.99±2.12	0.54±1.38
P178A	18.8±8.39	2.39±.63	1.27±0.90
Q179A	N/A	N/A	N/A
N181A	N/A	N/A	N/A
N182A	58.17±37.87	4.14±2.15	0.71±0.83
F183A	N/A	N/A	N/A
V184A	47.12±55.95	5.77±5.24	1.22±2.57
F186A	15.23±15.17	.77±.43	0.51±0.79
F190A	107.3±99.03	5.97±4.82	0.56±0