Table 1.
Effect of Diets Supplemented with Sorghum Leaf Sheath Red Dye on Some Biochemical Indices in Cisplatin (7 mg/kg i.p.) Administered Rats
| Treatment groups | ||||
|---|---|---|---|---|
| I | II | III | IV | |
| Average feed intake (g/rat/day) | 9.2±3.4a | 9.1±4.0a | 9.0±5.2a | 9.5±2.6a |
| Average weight gain/loss (%) | 5.9d | −2.8a | −1.6b | −1.2c |
| Plasma indices | ||||
| ALT (IU/L) | 61.1±3.6a | 70.3±11.0a | 57.6±10.6a | 53.6±7.9a |
| AST (IU/L) | 21.1±5.5b | 26.3±10.3b | 17.6±0.6a | 15.5±1.0a |
| Albumin (mg/dL) | 3.2±0.4b | 2.8±0.1a | 3.2±0.4b | 3.7±0.3c |
| Plasma enzymatic antioxidant indices | ||||
| Catalase activity (mmol H2O2 consumed/min/mg protein) | 180.0±20.0bc | 131.1±22.1a | 174.0±82.1b | 200.2±7.5c |
| GST activity (mmol CDNB conjugates formed/min/g protein) | 3.4±0.5d | 2.5±0.1a | 3.0±0.2c | 2.9±0.1b |
| SOD activity (Units/mg protein) | 90.2±0.5a | 61.1±0.5b | 140.4±1.3a | 200.3±1.2a |
| Plasma nonenzymatic antioxidant indices | ||||
| Vitamin C content (mmol/mg protein) | 9.4±0.4c | 4.6±0.3a | 6.0±0.1b | 5.7±0.2b |
| MDA content (mmol/100 g protein) | 1.1±0.6b | 8.2±1.9c | 0.9±0.2b | 0.6±0.2a |
| Plasma lipid profile (mg/dL) | ||||
| Triglycerides | 160.2±39.2a | 200.3±62.0c | 207.2±18.0c | 192.3±57.7b |
| Total cholesterol | 129.1±7.6a | 157.0±10.1c | 150.5±14.5c | 138.3±14.7b |
| LDL-cholesterol | 73.4±21.3a | 114.9±17.3b | 57.4±17.4a | 56.8±17.8a |
| HDL-cholesterol | 12.7±6.2b | 8.2±12.2a | 71.6±3.8d | 59.5±5.9c |
| Liver enzymatic antioxidant indices | ||||
| Catalase activity (mmol H2O2 consumed/min/mg protein) | 25.2±0.8b | 23.8±0.4a | 26.7±0.3b | 29.5±0.2c |
| GST activity (mmol CDNB conjugates formed/min/g protein) | 4.18±0.1c | 3.92±0.3a | 4.04±0.1b | 4.22±0.1c |
| SOD activity (Units/100 g protein) | 3.4±0.4b | 2.7±0.2a | 5.0±0.2c | 5.4±0.3c |
| Liver nonenzymatic antioxidant indices | ||||
| Vitamin C (mmol/mg protein) | 6.8±0.5a | 5.8±0.1a | 6.5±0.7a | 7.2±0.2a |
| MDA (mmol/mg protein) | 8.3±0.1a | 9.3±0.5b | 8.9±1.2b | 8.9±0.6b |
| GSH (mg/100 g protein) | 1.6±0.1b | 0.62±0.7a | 1.7±0.2b | 1.8±1.1b |
Values represent mean±standard deviation (n=6).
Values not sharing the same superscript letter on the same row are significantly (P<.05) different.
Adult male rats were divided into four groups of six animals each. Groups I (normal rats fed basal diet), group II (control rats administered cisplatin and fed basal diet), group III (cisplatin administered rats fed diet supplemented with 0.5% sorghum leaf sheath red dye), and group IV (cisplatin-administered rats fed diet supplemented with 1% sorghum leaf sheath red dye). The rats were maintained on these diets for 20 days before cisplatin administration. A single dose of cisplatin (7 mg/kg body weight, i.p.) was administered to the rats on the 20th day, and the experiment was terminated 3 days later. Plasma and liver biochemical indices were examined as evidence of cisplatin toxicity.
ALT, alanine aminotransferase; AST, aspartate aminotransferase; GSH, glutathione; GST, glutathione-S-transferase; HDL, high-density lipoprotein; LDL, low-density lipoprotein; MDA, malondialdehyde; SOD, superoxide dismutase.