Table 1.
Target gene | Primer | Sequencea(5′-3′) | Restriction site | Expected product (bp) |
---|---|---|---|---|
PCV2 ORF2 | ORF2fs | CTT AGT CGA CAT GAC GTA TCC AAG GAG | Sal I | 722 |
ORF2rs | CGG GAG TAC TAT TCA TTA AGG GTT AAG | Sca I | ||
Porcine IL-18 | pIL18fs | TAA GCG GCC GCA TGT ATA AGA TGC AGC T | Not I | 599 |
pIL18rs | CGT CTC GAG TCA AGT CAG TGT TG | Xho I | ||
PCV2 ORF1 | ORF1fs | TGG GTG TGG CAA AAG CAA ATG | 191 | |
ORF1rs | TAG TCT CAA CAG TCA AAG GAT |
The restriction enzyme sites used for the construction are underlined.
PCR, polymerase chain reaction; PCV2, porcine circovirus type 2.