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. 2014 Dec 1;27(10):521–528. doi: 10.1089/vim.2014.0036

Table 1.

Primers Used for PCR Amplification of Target Genes in This Study

Target gene Primer Sequencea(5′-3′) Restriction site Expected product (bp)
PCV2 ORF2 ORF2fs CTT AGT CGA CAT GAC GTA TCC AAG GAG Sal I 722
  ORF2rs CGG GAG TAC TAT TCA TTA AGG GTT AAG Sca I  
Porcine IL-18 pIL18fs TAA GCG GCC GCA TGT ATA AGA TGC AGC T Not I 599
  pIL18rs CGT CTC GAG TCA AGT CAG TGT TG Xho I  
PCV2 ORF1 ORF1fs TGG GTG TGG CAA AAG CAA ATG   191
  ORF1rs TAG TCT CAA CAG TCA AAG GAT    
a

The restriction enzyme sites used for the construction are underlined.

PCR, polymerase chain reaction; PCV2, porcine circovirus type 2.