Table 2.
Immunohistochemistry Detection Results and Mean Score in the Tissues of Pigs at Necropsy 28 Days Following Intranasal and Intramuscular Inoculations with PCV2
| No. of piglets with IHC detection positive/total | Mean scorea | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Group | Heart | Liver | Spleen | Lung | Lymph node | Heart | Liver | Spleen | Lung | Lymph node |
| pBudCE4.1-ORF2/IL18 | 0/5 | 0/5 | 0/5 | 1/5 | 1/5 | 0.0±0.00 | 0.0±0.00 | 0.0±0.00 | 0.2±0.45 | 0.4±0.72 |
| pBudCE4.1-ORF2 | 1/5 | 1/5 | 1/5 | 1/5 | 3/5 | 0.2±0.57 | 0.0±0.00 | 0.2±0.75 | 0.4±0.65 | 0.6±0.43 |
| pBudCE4.1 | 3/5 | 3/5 | 4/5 | 4/5 | 5/5 | 0.8±0.39 | 1.0±0.45 | 1.4±0.71 | 1.8±0.39* | 2.6±0.62* |
| PBS | 3/5 | 3/5 | 4/5 | 5/5 | 5/5 | 1.0±0.73 | 1.2±0.55 | 1.6±0.55 | 2.0±0.71* | 2.8±0.63* |
a
Values are the mean estimated amounts of the PCV2 antigen in the tissues (range: 0, no antigen detected; 3, high amounts of antigen).
*
p<0.05 (compared with pBudCE4.1-ORF2/IL18 or pBudCE4.1-ORF2).
IHC, immunohistochemistry; PBS, phosphate-buffered saline.