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. 2014 Dec 1;27(10):543–550. doi: 10.1089/vim.2014.0080

FIG. 3.

FIG. 3.

Expression of the MERS-CoV S protein in 293FT and BHK cells using antisera against the immunogens used in this study. Samples were derived from 293FT or BHK cells transiently transfected with pVRC-SY or pVRC8304 (indicated as Mock). (A) Western blot showing S-protein expression. 293FT cells were transfected with pVRC-SY(nCoV). Thirty-six hours later, cell lysates were prepared and Western blots were performed by using the indicated antiserum. Asterisks indicate the correct bands of S protein. (B) Immunofluorescence assay (IFA) of pVRC-SY(nCoV)-transfected BHK cells. Cells were fixed, permeabilized, and stained for either the S protein using the indicated antibody, or chromatin using a 4, 6-diamidino-2-phenylindole (DAPI) stain. The first row represents S protein, and the second row represents the chromatin. Cells were analyzed by confocal microscopy using a 100× objective, and representative images are shown.