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Neutralization assay to screen the rabbit antisera raised by peptide immunization. MERSpp was premixed 1:1 with the indicated dilution of sera at 37°C before inoculation into Huh-7 cells, and the infection efficacy was monitored at 48 h postinfection (Protocol I). The normalized neutralization was calculated as described in Materials and Methods. The experiment was carried out in triplicate; data from one representative case are shown. Error bars indicate the SE of the mean.
