. 2014 Dec 1;27(10):529–542. doi: 10.1089/vim.2014.0088

Table 1.

Sequence Information for Kompetitive Allele-Specific Polymerase Chain Reaction Assay Design

SNP ID	Genomic position^a	Allele	Allele labeled by FAM	Allele labeled by HEX	Flanking region
SNP1	chr6:3,989,214 to 3,989,415	CG/TA	CG	TA	AGAACGTAGTAGAGAAGAATTGAAACAGAAGATGGCTGGATTTTTATCTGAAGGACCAGCCTGCCAAGAGGAAAAAAAAAAAAGTCCTTCACGTTTCCAC[CG/TA]AAAGCTGTAATTTTTAGCTTTATTCAGGGTTTTCCGGCTTATCTCTGTCTTGCACAATCCTAGCGATCATCTACTTCAACCTTACCAYCCYTATTTACAG
SNP2	chr6:3,989,303 to 3,989,506	CCCC/TCCT	CCCC	TCCT	CACGTTTCCACYRAAAGCTGTAATTTTTAGCTTTATTCAGGGTTTTCCGGCTTATCTCTGTCTTGCACAATCCTAGCGATCATCTACTTCAACCTTACCA[CCCC/TCCT]TATTTACAGGAATTGCGTATGACACTCTGTGAGTAAACCCTTTGCTCTTTGCCTGGCCTTGGATATATATTGACTTTGTTACAGTACAAATGAAGAACAA

The design is based on the flanking regions of SNP1 and SNP2 in the chicken genomic MBL promoter sequences. The SNPs are marked with a forward slash (/) and surrounded by square brackets.

^{^a}

NC_006093.3.

MBL, mannose-binding lectin.