Table 1.
Antibodies for Immunohistochemistry
| Dilution | Supplier | |
|---|---|---|
| Primary antibodies | ||
| Rb anti-GFP | 1:300–500 | Molecular Probes |
| G anti-OCN | 1:100 | Santa Cruz |
| SH anti-E11 | 1:100 | Developmental Studies Hybridoma Bank |
| G anti-SDF-1 (C19) | 1:400 | Santa Cruz |
| Secondary antibodies | ||
| FITC-conjugated G anti-Rb | 1:200 | Jackson ImmunoResearch |
| Cy3-conjugated D anti-G | 1:200 | Jackson ImmunoResearch |
| Cy3-conjugated G anti-SH | 1:200 | Jackson ImmunoResearch |
| Biotin-conjugated D anti G | 1:200 | Vector Laboratories |
| Reagents | ||
| HRP-conjugated anti-Rb | 1:400 | Jackson ImmunoResearch |
| FITC-conjugated tyramide | 1:800 | Perkin Elmer |
| Biotin-conjugated tyramide | 1:400 | Perkin Elmer |
D, donkey; G, goat; HRP, horseradish peroxidase; OCN, osteocalcin; Rb, rabbit; SDF-1, stromal cell-derived factor-1; SH, syrian hamster.