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(a) Superdex 75 gel-filtration chromatogram of gel-filtration standards (Bio-Rad). (b) Superdex 75 gel-filtration chromatogram of PatG-DUF. (c) Standard curve (elution volume versus log MW). Interpolating from the standard curve, we can determine that PatG-DUF elutes from the gel-filtration column as a monomer.
