Table 1. Macromolecule-production information.

Source organism	FIPV strain 791146 (GenBank accession No. AF326575)
DNA source	Synthetic plasmid
Forward primer†	CGGGATCCAGCGGTCTGCGTAAAATG
Reverse primer‡	CCGCTCGAGTTACTGCAGATTAACGCCATAC
Cloning vector	pGEX-6P-1
Expression vector	pGEX-6P-1
Expression host	E. coli BL21 (DE3)
Complete amino-acid sequence of the construct produced	GPLGSSGLRKMAQPSGVVEPCIVRVAYGNNVLNGLWLGDEVICPRHVIASDTSRVINYENELSSVRLHNFSIAKNNAFLGVVSAKYKGVNLVLKVNQVNPNTPEHKFKSVRPGESFNILACYEGCPGSVYGVNMRSQGTIKGSFIAGTCGSVGYVLENGTLYFVYMHHLELGNGSHVGSNLEGEMYGGYEDQPSMQLEGTNVMSSDNVVAFLYAALINGERWFVTNTSMTLESYNAWAKTNSFTEIVSTDAFNMLAAKTGYSVEKLLECIVRLNKGFGGRTILSYGSLCDEFTPTEVIRQMYGVNLQ

^†Underlined sequence: BamHI site.

^‡Underlined sequence: XhoI site.