Table 2. Crystallization.
Method | Hanging-drop vapour diffusion |
Plate type | 16-well crystallization plate |
Temperature (K) | 291 |
Protein concentration (mgml1) | 8 |
Buffer composition of protein solution | 10mM HEPES pH 7.5, 150mM NaCl, 1mM DTT |
Composition of reservoir solution | 0.2M zinc acetate dihydrate, 0.1M sodium cacodylate trihydrate pH 6.5, 14%(w/v) polyethylene glycol 8000 |
Volume and ratio of drop | 1.0l + 1.0l |
Volume of reservoir (l) | 200 |