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. 2014 Nov 14;70(Pt 12):1612–1615. doi: 10.1107/S2053230X14022390

Table 2. Crystallization.

Method Hanging-drop vapour diffusion
Plate type 16-well crystallization plate
Temperature (K) 291
Protein concentration (mgml1) 8
Buffer composition of protein solution 10mM HEPES pH 7.5, 150mM NaCl, 1mM DTT
Composition of reservoir solution 0.2M zinc acetate dihydrate, 0.1M sodium cacodylate trihydrate pH 6.5, 14%(w/v) polyethylene glycol 8000
Volume and ratio of drop 1.0l + 1.0l
Volume of reservoir (l) 200