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(a) Size-exclusion chromatogram and SDS–PAGE analysis (inset) of BabA25–460 run on Superdex 75 10/300 (GE Healthcare). (b) CD spectrum of BabA25–460 in phosphate buffer (50 mM KH2PO4/K2HPO4 pH 8.0) reveals a predominantly α-helical secondary-structure content.
