(a) Image of BabA25–460–Nb-ER19 crystals and SDS–PAGE of dissolved crystals alongside purified BabA25–460 (inset, labelled C and P, respectively; *, BabA25–460; **, Nb-ER19). (b) Representative diffraction image (0.2° rotation, 0.2 s exposure at 20% transmission) from a crystal of BabA25–460 in complex with Nb-ER19 obtained by the sitting-drop vapour-diffusion method.