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. 2014 Nov 14;70(Pt 12):1636–1639. doi: 10.1107/S2053230X14023243

Table 1. Macromolecule-production information.

Source organism X. campestris pv. campestris (strain 8004)
DNA source Genomic
Forward primer (NdeI site + His6 tag) ATCATATG CACCATCACCATCACCATAGCACTGCAACCAACCCGTTG
Reverse primer (BamHI site) TAGGATCCTTATTCCGGATCGCGCAGCTGTAAC
Cloning vector pET-24a
Expression vector pET-24a
Expression host E. coli BL21(DE3)pLysS
Complete amino-acid sequence of the construct produced MHHHHHHSTATNPLDLDVCAREPIHIPGLIQPYGVLLVIDPADGRIVQASTTAADLLGVPMAALLGMPYTQVLTLPEAQPFAVDDQPQHLMHAEVRFPQRATPPASAWVAAWHLYPQQWLVEMEPRDARLLDVTLREAMPLLRSVERDPGIAEAAVRVAKGLRSLIGFDRVMIYRFDEEWNGDIIAEARKPELEAYLGLHYPASDIPAQARALYLRNRVRQIADVGYQPSPIQPTVHPQLGTPVDLSDVSLRSVSPVHLEYLANMGVTATLVASIVVNDALWGLISCHHYSPHFTNHAMRDVTDAVARTLAGRIGALQAVARARLESVLLTVREKLITDFNDAEHMTVELLDDMAPDLMDVVDADGVAIFHGNDISRHGTTPDVAALRRIRDHIESEHHEALREDAVGALHVDAIGEVFPELADLAPLAAGFIFVPLMPQSRSALLWTRREQIQQIKWAGNPQLAKLEDIPNSRLSPRKSFDLWQQTVRGRARRWSPLHLESARSLRVLIELMERKRFQQDFTLLEASLSRLRDGVAIIERGTANAAHRLLFVNTAFADVCGSDVAELIGRELQTLYASDAPRANVELLQDALRNGRAAYVTLPLQVSDGAPVYRQFHLEPLPSPSGVTAHWLLQLRDPE