| Method |
Vapour diffusion, hanging drop |
| Plate type |
Hampton Research VDX (24-well) |
| Temperature (C) |
21 |
| Light condition |
Experiment performed in the dark |
| Protein concentration (mgml1) |
11 |
| Buffer composition of protein solution |
10mM Tris, 25mM sodium chloride pH 7.6 |
| Composition of reservoir solution |
12%(w/v) PEG 4000, 0.1M Tris, 0.2M sodium acetate pH 8.3 |
| Volume and ratio of drop |
2l, 1:1 |
| Volume of reservoir (l) |
500 |
| Crystallization time (harvesting of the best crystals) |
3 weeks |
| Maximum crystal dimensions (mm) |
0.5 0.3 0.2 |
| Cryoprotectant solution |
Mother liquor supplemented with 25%(w/v) PEG 400 |
