Table 1. Macromolecule-production information.

Additional residues are underlined.

Source organism	A. nidulans FGSC A4
DNA source	The genome of A. nidulans
Rpf2(1304)
Expression vector	pET-28a (Novagen)
Cloning site	NdeIXhoI
Additional residues	Downstream box†, N-terminal His tag, TEV cleavage site
Expression host	E. coli B834 (DE3) pRARE2
Complete amino-acid sequence of the construct produced	MNHKHHHHHHSSGENLYFQGHMLREVKPKNPRTARILKAKEPQLIEGAKRVLLLHGSKCPTPLHTVLKVFHSLTTPHSVLFHKKNENIHPFESTESLEFLANKNDCGMVIFGSSNKKRPNCLTIARIFDSKVLDMAELLLLPDANGEGIPEMNRLSMHVAIGLRPLMLFSGSAWDDTTSTTHTMLKSMLVDLFKGETSDKIDVEGLQYALMVGAEEPTAGLAPIIHLRWYKIVTKRSGHKLPRVELEEIGPKLDFKVGRIQEAPRDVMKEAMKQGKKPNEEIKNKKNIGIDLIGDKVGRVHLAKQDLGGLQTRKMKGLKRRAG
Rrs1(1218) (full length)
Expression vector	pCDF Duet-1 (Novagen)
Cloning site	NdeINcoI
Additional residues	Downstream box†
Expression host	E. coli B834 (DE3) pRARE2
Complete amino-acid sequence of the construct produced	MNHTMANSETEAKSTIKPKPERLPITVSKPTPYTFDLGHLLANDPNPLELPKSEPLNASLKATARDGVQSLLNQLLTTCPITSSQQGVLLTLPAPSTILPRHKPLPTPKPPTKWELFARKKGIGKYSNKPGAALADKERRKKLVYDEESGEWVPRWGYKGKNKDDDQWLVEVKEKDWKKEEEAAAKGSSIRGMSRAERKERIRRNERKMRANERNSRKSGGK

^†The downstream box is a sequence element that enhances translation (Sprengart et al., 1996 ▶).