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| Method | Hanging drop |
| Plate type | 24-well Linbro plate |
| Temperature (C) | 18 |
| Protein concentration (mgml1) | 10 |
| Buffer composition of protein solution | 20mM TrisHCl pH 8, 300mM NaCl, 1%(v/v) OG |
| Composition of reservoir solution | 0.05M glycine pH 9, 50%(v/v) PEG 400, 5% glycerol, 0.05%(v/v) CYMAL-6, 0.05%(v/v) n-nonyl--D-thiomaltopyranoside, 0.05%(v/v) dodecyl--D-maltoside |
| Volume and ratio of drop | 2l protein solution, 2:1 ratio |
| Volume of reservoir (l) | 500 |
