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| Method | Hanging-drop vapour diffusion |
| Plate type | 24-well |
| Temperature (K) | 293 |
| Protein concentration (mgml1) | 10 |
| Buffer composition of protein solution | 20mM TrisHCl pH 8.0, 50mM NaCl |
| Composition of reservoir solution | 14% PEG 8000, 0.1M imidazole pH 8.0, 0.2M calcium acetate |
| Volume and ratio of drop | 4l (1:1) |
| Volume of reservoir (ml) | 1 |
