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Purification of PhyHT on a gel-filtration column and SDS–PAGE analysis. (a) 12% SDS–PAGE stained with Coomassie Brilliant Blue. Lane M, protein marker (labelled in kDa); lanes 1–4, PhyHT corresponding to peak 2 on the gel-filtration profile (b). (b) Purification profile of PhyHT, which eluted as a symmetrical peak from the Superdex G200 SEC column (peak 2).
