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. 2014 Nov 28;70(Pt 12):1697–1700. doi: 10.1107/S2053230X14025278

Table 1. Macromolecule-production information.

Source organism B. pseudomallei
DNA source Chemically synthesized (GenScript, USA)
Cloning vector pUC57
Expression vector pET-28b
Expression host E. coli strain BL21-Rosetta gami (DE3)
Complete amino-acid sequence of the construct produced MGSSHHHHHHSSGLVPRGSHMAGNLVIVCRDQDADAFDQLMQEYGSFQTRLSSTAWYLNMNIVPETLQEDILERVGKYTTLYIFEATSVTYNTIDSNAAETLSTLFGE

The His-tag fusion sequence at the N-terminus of recombinant BPSL1038 is underlined.