| Method |
Sitting-drop vapour diffusion for initial crystal screening and hanging-drop vapour diffusion for crystal optimization |
| Plate type |
96-well MRC plates for initial crystal screening and 24-well plates for crystal optimization |
| Temperature (K) |
293 |
| Protein concentration (mgml1) |
10 |
| Buffer composition of protein solution |
25mM TrisHCl pH 7.5, 100mM sodium chloride, 20mM -mercaptoethanol |
| Composition of reservoir solution |
0.1M sodium acetate pH 4.6, 2M sodium formate |
| Volume and ratio of drop |
1.0l for initial crystal screening and 2.0l for optimization; 1:1 protein:reservoir solution |
| Volume of reservoir |
80l for initial crystal screening and 1.0ml for optimization |
