Table 3. Data-collection and refinement parameters.
Diffraction data were obtained in situ from lysozyme crystals that were grown using 2.5nl protein solution and 2.5nl precipitant solution. Data were obtained from 12 crystals that were grown on a plate that was prepared with a plate lid and from 12 crystals that were grown on a plate that was prepared without a plate lid. The top of the table shows the data-collection statistics for each group of 12 data sets (average standard deviation). The bottom of the table shows the merging statistics and refinement statistics after merging each group of 12 data sets.
| With lid | Without lid | |
|---|---|---|
| Crystal information | ||
| No. of crystals | 12 | 12 |
| Space group | P43212 | P43212 |
| Unit-cell parameters () | ||
| a = b | 79.573 0.118 | 79.563 0.085 |
| c | 37.854 0.075 | 37.843 0.099 |
| Data-collection statistics (average of 12 unmerged data sets) | ||
| Resolution () | 1.44 0.08 | 1.55 0.09 |
| Unique reflections | 18255 3186 | 15060 2525 |
| R sym (%) | 5.8 0.9 | 7.6 1.4 |
| Merging statistics (merged data) | ||
| Resolution () | 1.4 | 1.5 |
| Unique reflections | 24370 | 19689 |
| R merge (%) | 9.6 | 11.6 |
| Refinement statistics (merged data) | ||
| R work (%) | 15.1 | 14.6 |
| R free (%) | 16.8 | 17.1 |
| R.m.s.d., bond lengths () | 0.029 | 0.027 |
| R.m.s.d., bond angles () | 2.53 | 2.33 |