Table 2.
IC50 values of doxorubicin and doxorubicin-conjugated phages on MICA-expressing cell lines
| Cell lines | IC50 (μM) doxorubicin | IC50 (μM) Dox-phage | IC50 (μM) Dox-phage after blocking MICA | MFI of MICA | Ratio Dox-phage |
|---|---|---|---|---|---|
| KKU-M214* | 0.2896 | 0.1775 | 0.2327 | 5.77 | 1.6315 |
| KKU-M213* | 0.0103 | 0.0017 | 0.0086 | 4.05 | 6.0588 |
| KKU-M055* | 5.0000 | 5.3448 | 5.3328 | 1.29 | 0.7923 |
| KKU-M139 | 0.0810 | 0.0258 | 0.0689 | 8.89 | 3.1395 |
| KKU-MMNK1 | 0.3155 | 0.0948 | 0.2741 | 2.63 | 3.3280 |
| Hela** | 0.0830 | 0.0150 | 0.0793 | 25.00 | 5.5333 |
Notes: IC50 was calculated by CalcuSyn Demo program.
*
Cholangiocarcinoma cell lines
**
cervical cancer cell lines.
Abbreviations: Dox-phage, Dox-carrying phage-displaying anti-MICA; MFI, mean fluorescence intensity; MICA, major histocompatibility complex class I chain-related A; IC50, half maximal inhibitory concentration.