Figure 2.

Increased activity of 1ea. A) Increased inhibition of PI(3,4)P2/Akt-PH domain binding by 1ea.Lipid overlay experiment was performed as performed as described in.³ B) Inhibition of S6 phopshorylation by 1ea. U87MG or A2780 cells were treated with indicated concentrations of inhibitors for 7 hr, followed by Western blotting using phopsho-S6 and total S6 antibodies.C) Increased cytotoxicity of 1ea compared to DM-PIT-1 in multiple cell types. Cells were treated with indicated concentrations of inhibitors alone or in combination with 1 μg/ml TRAIL for 24 hr. Cell viability was determined using CellTiter-Glo assay. D) 1ea efficiently blocks migration of A2780 cells. Wound healing assay was performed as previously described.⁴ Cell monolayers were treated with indicated concentrations of inhibitors, followed by a scratch wound. Size of the cell free area was photographed at indicated periods of time. Quantification of open wound area is shown on the right.