Fig. 5.

Post-thaw motility (a) and adjusted motility index (AMI) (b) of rhesus monkey ejaculated sperm samples frozen with 3% glycerol and without glycerol in TEST with 20% egg yolk, and thawed with three different methods: in a 37 °C water bath for 30 s, at 23 °C room temperature air for 10 min, and in a 70 °C water bath for 5 s. Samples were cooled at 220 °C/min. Post-thaw motility and AMI were estimated after incubation at 37 °C in 5% CO₂ in air for 0 h (left bars of the same color) and 1 h (right bars of the same color).