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. 2014 Dec 8;9(12):e114374. doi: 10.1371/journal.pone.0114374

Figure 5. Immune responses of atopic Nc/Nga mice immunized with MVA and Dryvax.

Figure 5

Mock-sensitized Nc/Nga mice were inoculated using a vaccination stamp trans-dermally (t.d.) or intra-muscularly (i.m.) with 108 PFU of a purified stock of MVA or t.d. with 107 PFU of a purified stock of Dryvax, and sacrificed 3 weeks later. (A) Levels of total serum IgG1 and IgG2a antibodies specific to VACV were determined by ELISA (dilution 1∶1,000); median values are indicated. Results of 2 independent experiments. (B) Neutralization capacity of mice sera expressed as number of plaques detected after a 12-h incubation of the input virus with the individual sera; see Materials and Methods section 6 for experimental details. Median values of the resulting numbers of plaques are indicated. Results of 1–2 independent experiments. *P<0.05, **P<0.01.