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. 2014 Dec 8;9(12):e112874. doi: 10.1371/journal.pone.0112874

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© 2014 Ezure et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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After synthesis of the membrane proteins in the cell-free system in the presence of FluoroTect Green_Lys tRNA, the membrane fraction was precipitated and separated by SDS/PAGE. (A) 10% polyacrylamide gel; lane 1, AtKC1 (plant, K⁺ channel, 6 transmembrane segments (TMS)); lane 2, KAT1 (plant, K⁺ channel, 6 TMS); lane 3, KAT2 (plant, K⁺ channel, 6 TMS); lane 4, GORK (plant, K⁺ channel, 6 TMS); lane 5, AKT1 (plant, K⁺ channel, 6 TMS); lane 6, NhaA (bacteria, Na⁺/H⁺ antiporter, 10 TMS); Lane 7, AspT (bacteria, aspartate: alanine antiporter, 10 TMS); lane 8, MsbA (bacteria, ABC transporter, 12 TMS). (B) 15% polyacrylamide gel; lane 9, Sec61 β(human, 1 TMS).