Table 2. Differentially phosphorylated proteins identified from the enriched proteins by 2-DE-LC-MS/MS between the selenate-treated and untreated N2aSW cells.

Spot No.a	Protein abbreviation	Protein nameb	score	number of peptides matched (searched)	Mass (kDa)/pIc	Fold Expd	Fold Phe
1	VINC	Vinculin	8384	233	117215/5.77	2.16	2.5
2	VINC	Vinculin	16790	576	117215/5.77	2.83	2.39
3	EF2	Elongation factor 2	17323	578	96222/6.41	1.29	2.26
4	EF2	Elongation factor 2	19977	631	96222/6.41	1.6	2.04
5	SYK	Lysine—tRNA ligase	4855	243	68253/5.65	1.12	2.05
6	HSP7C	Heat shock cognate 71 kDa protein	7263	273	71055/5.37	2.28	3.12
7	TCPG	T-complex protein 1 subunit gamma	6154	252	61162/6.28	0.65	2.17
8	DPYL2	Dihydropyrimidinase-related protein 2	1499	48	62638/5.95	1.42	2.09
9	SYYC	Tyrosine—tRNA ligase, cytoplasmic	8843	379	59410/6.57	1.61	3.11
10	ATPB	ATP synthase subunit beta, mitochondrial	10434	301	56265/5.19	0.62	0.49
11	IF4A1	Eukaryotic initiation factor 4A-I	4468	139	46353/5.32	0.41	0.42
12	SEPT2	Septin-2	448	15	41727	2.31	2.81
13	HNRH1	Heterogeneous nuclear ribonucleoprotein H	1813	54	49454/5.89	2.55	2.22
14	HSP7C	Heat shock cognate 71 kDa protein	3055	90	71055/5.37	1.44	2.44
15	ALDOA	Fructose-bisphosphate aldolase A	930	31	39787	2.52	2.76
16	TWF2	Twinfilin-2	1359	33	39674/6.33	2.19	2.26
17	EEF1-delta	Elongation factor 1-delta	4947	133	31387	0.29	2.26
18	ALDR	Aldose reductase	1583	82	36052/6.71	1.6	0.41
19	ENOA	Alpha-enolase	2275	72	47453/6.37	1.68	2.16
20	PRPS1	Ribose-phosphate pyrophosphokinase 1	1540	54	35325	2.01	2.55
21	PRS6B	26S protease regulatory subunit 6B	1221	41	47493	2.29	3.55
23	5NT3B	7-methylguanosine phosphate-specific 5'-nucleotidase	1243	42	34688	2.58	3.31
24	PSA1	Proteasome subunit alpha type-1	1741	55	29813/6	0.17	2.37
25	KPYM	Pyruvate kinase isozymes M1/M2	1471	44	58378/7.18	2.67	2.98
26	MTAP	S-methyl-5'-thioadenosine phosphorylase	3747	156	31612/6.71	2.92	3.37
27	ACTBL	Beta-actin-like protein 2	2027	75	42319	1.87	2.05
28	CYBP	Calcyclin-binding protein	676	33	26608/7.63	1.82	2.24
29	SGTA	Small glutamine-rich tetratricopeptide repeat-containing protein alpha	1320	29	34529/4.99	2.07	0.46
31	PRDX6	Peroxiredoxin 6	873	33	24925/5.98	1.3	3.66
32	PSA2	Proteasome subunit alpha type-2	1369	53	26024/6.92	1.25	2.18
34	GDIR1	Rho GDP-dissociation inhibitor 1	2240	86	23450/5.12	1.69	0.23
35	EMC8	ER membrane protein complex subunit 8	658	20	23790/5.72	0.42	0.35
36	SERB	Phosphoserine phosphatase	853	30	25308	0.19	0.21
38	PSB2	Proteasome subunit beta type-2	941	28	23063	2.19	2.38
39	BLVRB	Flavin reductase (NADPH)	427	15	22297/6.49	2.23	2.98
40	ACTB	Actin, cytoplasmic 1	205	9	42052	1.69	4.35
41	PSB6	Proteasome subunit beta type-6	3466	119	25591/4.97	1.36	2.47
43	PRDX2	Peroxiredoxin-2	1628	65	21936/5.20	0.65	0.4
47	KPYM	Pyruvate kinase isozymes M1/M2	757	36	58378/7.18	3.64	3.26
48	NDKA	Nucleoside diphosphate kinase A	8479	396	17311/6.84	3.2	2.58
49	MGDP1	Magnesium-dependent phosphatase 1	1305	39	18628/6.29	4.33	4.05
50	MGN	Protein mago nashi homolog	790	36	17210/5.74	0.92	2.49
52	PFD5	Prefoldin subunit 5	852	28	17402	5.27	2.72
53	HINT1	Histidine triad nucleotide-binding protein 1	2916	119	13882/6.36	2.4	2.53
54	LEG1	Galectin-1	837	21	15198	5.32	2.57

^aProtein IDs were assigned manually.

^bProtein names were identified by MS.

^cTheoretical molecular weight and isoelectrical point of the protein(s).

^dThe ratio in spot density from the Rubby stained gel of the selenate treated cells compared to the control (untreated cells).

^eThe ratio in spot density from the Pro-Q diomand stained gel of the selenate treated cells compared to the control (untreated cells).