Table 2. Differentially phosphorylated proteins identified from the enriched proteins by 2-DE-LC-MS/MS between the selenate-treated and untreated N2aSW cells.
Spot No.a | Protein abbreviation | Protein nameb | score | number of peptides matched (searched) | Mass (kDa)/pIc | Fold Expd | Fold Phe |
1 | VINC | Vinculin | 8384 | 233 | 117215/5.77 | 2.16 | 2.5 |
2 | VINC | Vinculin | 16790 | 576 | 117215/5.77 | 2.83 | 2.39 |
3 | EF2 | Elongation factor 2 | 17323 | 578 | 96222/6.41 | 1.29 | 2.26 |
4 | EF2 | Elongation factor 2 | 19977 | 631 | 96222/6.41 | 1.6 | 2.04 |
5 | SYK | Lysine—tRNA ligase | 4855 | 243 | 68253/5.65 | 1.12 | 2.05 |
6 | HSP7C | Heat shock cognate 71 kDa protein | 7263 | 273 | 71055/5.37 | 2.28 | 3.12 |
7 | TCPG | T-complex protein 1 subunit gamma | 6154 | 252 | 61162/6.28 | 0.65 | 2.17 |
8 | DPYL2 | Dihydropyrimidinase-related protein 2 | 1499 | 48 | 62638/5.95 | 1.42 | 2.09 |
9 | SYYC | Tyrosine—tRNA ligase, cytoplasmic | 8843 | 379 | 59410/6.57 | 1.61 | 3.11 |
10 | ATPB | ATP synthase subunit beta, mitochondrial | 10434 | 301 | 56265/5.19 | 0.62 | 0.49 |
11 | IF4A1 | Eukaryotic initiation factor 4A-I | 4468 | 139 | 46353/5.32 | 0.41 | 0.42 |
12 | SEPT2 | Septin-2 | 448 | 15 | 41727 | 2.31 | 2.81 |
13 | HNRH1 | Heterogeneous nuclear ribonucleoprotein H | 1813 | 54 | 49454/5.89 | 2.55 | 2.22 |
14 | HSP7C | Heat shock cognate 71 kDa protein | 3055 | 90 | 71055/5.37 | 1.44 | 2.44 |
15 | ALDOA | Fructose-bisphosphate aldolase A | 930 | 31 | 39787 | 2.52 | 2.76 |
16 | TWF2 | Twinfilin-2 | 1359 | 33 | 39674/6.33 | 2.19 | 2.26 |
17 | EEF1-delta | Elongation factor 1-delta | 4947 | 133 | 31387 | 0.29 | 2.26 |
18 | ALDR | Aldose reductase | 1583 | 82 | 36052/6.71 | 1.6 | 0.41 |
19 | ENOA | Alpha-enolase | 2275 | 72 | 47453/6.37 | 1.68 | 2.16 |
20 | PRPS1 | Ribose-phosphate pyrophosphokinase 1 | 1540 | 54 | 35325 | 2.01 | 2.55 |
21 | PRS6B | 26S protease regulatory subunit 6B | 1221 | 41 | 47493 | 2.29 | 3.55 |
23 | 5NT3B | 7-methylguanosine phosphate-specific 5'-nucleotidase | 1243 | 42 | 34688 | 2.58 | 3.31 |
24 | PSA1 | Proteasome subunit alpha type-1 | 1741 | 55 | 29813/6 | 0.17 | 2.37 |
25 | KPYM | Pyruvate kinase isozymes M1/M2 | 1471 | 44 | 58378/7.18 | 2.67 | 2.98 |
26 | MTAP | S-methyl-5'-thioadenosine phosphorylase | 3747 | 156 | 31612/6.71 | 2.92 | 3.37 |
27 | ACTBL | Beta-actin-like protein 2 | 2027 | 75 | 42319 | 1.87 | 2.05 |
28 | CYBP | Calcyclin-binding protein | 676 | 33 | 26608/7.63 | 1.82 | 2.24 |
29 | SGTA | Small glutamine-rich tetratricopeptide repeat-containing protein alpha | 1320 | 29 | 34529/4.99 | 2.07 | 0.46 |
31 | PRDX6 | Peroxiredoxin 6 | 873 | 33 | 24925/5.98 | 1.3 | 3.66 |
32 | PSA2 | Proteasome subunit alpha type-2 | 1369 | 53 | 26024/6.92 | 1.25 | 2.18 |
34 | GDIR1 | Rho GDP-dissociation inhibitor 1 | 2240 | 86 | 23450/5.12 | 1.69 | 0.23 |
35 | EMC8 | ER membrane protein complex subunit 8 | 658 | 20 | 23790/5.72 | 0.42 | 0.35 |
36 | SERB | Phosphoserine phosphatase | 853 | 30 | 25308 | 0.19 | 0.21 |
38 | PSB2 | Proteasome subunit beta type-2 | 941 | 28 | 23063 | 2.19 | 2.38 |
39 | BLVRB | Flavin reductase (NADPH) | 427 | 15 | 22297/6.49 | 2.23 | 2.98 |
40 | ACTB | Actin, cytoplasmic 1 | 205 | 9 | 42052 | 1.69 | 4.35 |
41 | PSB6 | Proteasome subunit beta type-6 | 3466 | 119 | 25591/4.97 | 1.36 | 2.47 |
43 | PRDX2 | Peroxiredoxin-2 | 1628 | 65 | 21936/5.20 | 0.65 | 0.4 |
47 | KPYM | Pyruvate kinase isozymes M1/M2 | 757 | 36 | 58378/7.18 | 3.64 | 3.26 |
48 | NDKA | Nucleoside diphosphate kinase A | 8479 | 396 | 17311/6.84 | 3.2 | 2.58 |
49 | MGDP1 | Magnesium-dependent phosphatase 1 | 1305 | 39 | 18628/6.29 | 4.33 | 4.05 |
50 | MGN | Protein mago nashi homolog | 790 | 36 | 17210/5.74 | 0.92 | 2.49 |
52 | PFD5 | Prefoldin subunit 5 | 852 | 28 | 17402 | 5.27 | 2.72 |
53 | HINT1 | Histidine triad nucleotide-binding protein 1 | 2916 | 119 | 13882/6.36 | 2.4 | 2.53 |
54 | LEG1 | Galectin-1 | 837 | 21 | 15198 | 5.32 | 2.57 |
Protein IDs were assigned manually.
Protein names were identified by MS.
Theoretical molecular weight and isoelectrical point of the protein(s).
The ratio in spot density from the Rubby stained gel of the selenate treated cells compared to the control (untreated cells).
The ratio in spot density from the Pro-Q diomand stained gel of the selenate treated cells compared to the control (untreated cells).